Rapid PCR-lateral flow assay for the onsite detection of Atlantic white shrimp

https://doi.org/10.1016/j.fochms.2023.100164Get rights and content
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Highlights

  • PCR-lateral flow assay for the detection of Atlantic white shrimp.

  • Standardized assay was validated using a miniaturized PCR machine.

  • Assay suited for onsite testing of raw, cooked, or processed shrimp samples.

Abstract

The Atlantic white shrimp (Litopenaeus setiferus) is of great economic importance to the United States and risk being substituted with imported species due to a shortage in domestic production. To improve the current methods used for the identification of the Atlantic white shrimp species, we designed and validated a robust multiplex PCR-lateral flow assay for the onsite identification of L. setiferus. The standardized assay was validated using a miniaturized, low-cost PCR instrument with 68 shrimp, prawn, and fish samples, spread over fourteen seafood species. L. setiferus was simultaneously amplified by the multiplex assay to give three visual bands, which distinguished it from other species having either one or two bands on the dipstick. The standardized assay showed 100% inclusivity for target L. setiferus samples, 100% exclusivity for non-target samples and can be completed in less than two hours. The assay standardized in this study can be used for onsite testing of L. setiferus samples at processing facilities, restaurants, and wholesalers' facilities.

Keywords

Internal amplification control
Lateral flow
Litopenaeus setiferus
Species substitution
White shrimp

Abbreviations

SIMP
Seafood Import Monitoring Program
IAC
Internal Amplification Control
DiGN
Digoxigenin
HRM
High-Resolution Melting

Data availability

No data was used for the research described in the article.

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